Статья

Development of elite indica rice lines with wide spectrum of resistance to Thai blast isolates by pyramiding multiple resistance QTLs

Tanee SreewongchaıDepartment of Agronomy, Faculty of Agriculture, Kasetsart University, Bangkok, 19000, Thailand, E-mail: [email protected]Theerayut ToojindaRice Gene Discovery Unit, National Center for Genetic Engineering and Biotechnology (BIOTEC), Kasetsart University, Khampengsen Campus, Nakhornpathom, 73140, ThailandN. ThanintornRice Gene Discovery Unit, National Center for Genetic Engineering and Biotechnology (BIOTEC), Kasetsart University, Khampengsen Campus, Nakhornpathom, 73140, ThailandChatchai KosawangRice Gene Discovery Unit, National Center for Genetic Engineering and Biotechnology (BIOTEC), Kasetsart University, Khampengsen Campus, Nakhornpathom, 73140, ThailandApichart VanavichitRice Gene Discovery Unit, National Center for Genetic Engineering and Biotechnology (BIOTEC), Kasetsart University, Khampengsen Campus, Nakhornpathom, 73140, ThailandDidier TharreauCIRAD, Campus international de Baillarguet, Montpellier, Cedex5, FranceP. SirithunyaFaculty of Science And Agricultural Technology, Rajamangala University of Technology Lanna, Chiangmai, 50300, Thailand

2009en

ABI

Аннотация

With 1 figure and 2 tables Abstract The rice varieties IR64 and Jao Hom Nin (JHN) demonstrated a broad‐spectrum resistance against the rice blast pathogens in Thailand. A genomic investigation unravelled many resistance genes residing on four genomic regions, chromosome 2 and 12 in IR64 and 1 and 11 in JHN. A cross between these varieties was made to combine resistance genes into a single genotype. Marker‐assisted selection (MAS) was employed to identify F 2 and F 3 plants carrying a combination of four resistance QTLs in a homozygous fusion. Flanking markers RM212/RM319 and RM144/RM139 to blast resistant QTLs on chromosome 1 and 11 in JHN rice and tightly‐linked markers RM208 and RM179 to blast resistant QTLs on chromosome 2 and 12 in IR64 rice variety were used for MAS. The stepwise MAS screening was brought in as a strategy to provide a cost‐saving and minimum number of PCR performing to select resistant genotypes. F 4 generation, lines carrying all resistant QTLs show a broader spectrum of resistance against 11 representatives of Thai blast pathogen isolates.

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Идентификаторы

DOI: 10.1111/j.1439-0523.2009.01669.x

Цитирования и источники

Цитирований: 2Использованных источников: 0

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