Cloning and characterization of a cotton lipid transfer protein gene specifically expressed in fiber cells
Din-Pow MaDepartment of Biochemistry and Molecular Biology, Box 9650, Mississippi State University, Mississippi, MS 39762 USAHsi-Chou LiuDepartment of Biochemistry and Molecular Biology, Box 9650, Mississippi State University, Mississippi, MS 39762 USAHingpoh TanDepartment of Biochemistry and Molecular Biology, Box 9650, Mississippi State University, Mississippi, MS 39762 USARoy G. CreechDepartment of Plant and Soil Sciences, Mississippi State University, Mississippi State, MS 39762, USAJohnie N. JenkinsCrop Science Research Laboratory, USDA/ARS, Mississippi State, MS 39762, USAYung‐Fu ChangDiagnostic Laboratory, New York State College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
1997en
ABI
Аннотация
A cotton genomic library was screened using a fiber-specific cDNA (GH3) encoding a lipid transfer protein (LTP). One genomic clone (1.7 kb DNA insert) containing the Ltp gene (Ltp6) was sequenced and characterized. The Ltp6 contains an open reading frame of 360 bp, which is interrupted by a single intron (136 bp) located in the region corresponding to the C-terminal of the protein. The derived amino-acid sequence of LTP6 is 64% homologous to that of GH3. Like the GH3 gene, the Ltp6 is specifically expressed in fiber cells in a temporal manner. However, its expression level is lower than that of GH3.
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