A Modified Micro-Drop Bioassay Using Dwarf Rice for Detection of Femtomol Quantities of Gibberellins

The sensitivity of the micro-drop assay with dwarf rice (Oryza sativa L., cv. Tan-ginbozu and cv. Waito-Q to gibberellins (GAs) was increased conspicuously by the use of assay plants that has been treated with uniconazole (S-3307), an inhibitor of the biosynthesis of GAs. The Tan-ginbozu plants treated with S-3307 responded to 10 fmol/plant of GA3 (ca. 3.5 pg/plant) and to 30 fmol/plant of gibberellins A1, A4, A7, A19 and A20. Waito-C plants treated with S-3307 responded to 10 fmol of GA3 and to 30 fmol/plant of gibberellins A1, A4 and A7. Gibberellins A9, A19 and A20 had much less of an effect on the treated Waito-C plants than did gibberellins A1, A3, A4 and A7. Furthermore, treatment with S-3307 counteracted the inhibition of growth of both cultivars by abscisic acid. Thus, the modified micro-drop assay should prove very useful for the detection of minute amounts of GAs in plant extracts.

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