The Production of Cellulases

Many fungi are cellulolytic, but only a few produce cell-free enzymes that will attack solid cellulose. Trichoderma viride grown on cellulose medium produces a stable cellulase complex including C1. This enzyme is capable of extensive degradation of solid celluloses. Conditions for producing high yields of the enzyme in shake flasks and in a laboratory fermenter are described. Filtrates from these cultures readily hydrolyzed nine cellulose substrates of varying resistance. It is suggested that such culture filtrates could be used to hydrolyze waste cellulose. The hydrolyzate could be used to produce single cell protein or some other fermentation product.

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