Статья

Pattern of Auxin and Cytokinin Responses for Shoot Meristem Induction Results from the Regulation of Cytokinin Biosynthesis by AUXIN RESPONSE FACTOR3

Zhi Juan ChengState Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, China (Z.J.C., L.W., W.S., Y.Zhang, C.Z., Y.H.S., W.L., T.T.S., X.Y.Z., X.G.L., X.S.Z.); Section of Cell and Developmental Biology, University of California San Diego, La Jolla, California 92093–0116 (Y.C., Y.Zhao); and State Key LaboraLiang WangState Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, China (Z.J.C., L.W., W.S., Y.Zhang, C.Z., Y.H.S., W.L., T.T.S., X.Y.Z., X.G.L., X.S.Z.); Section of Cell and Developmental Biology, University of California San Diego, La Jolla, California 92093–0116 (Y.C., Y.Zhao); and State Key LaboraWei SunState Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, China (Z.J.C., L.W., W.S., Y.Zhang, C.Z., Y.H.S., W.L., T.T.S., X.Y.Z., X.G.L., X.S.Z.); Section of Cell and Developmental Biology, University of California San Diego, La Jolla, California 92093–0116 (Y.C., Y.Zhao); and State Key LaboraYan ZhangState Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, China (Z.J.C., L.W., W.S., Y.Zhang, C.Z., Y.H.S., W.L., T.T.S., X.Y.Z., X.G.L., X.S.Z.); Section of Cell and Developmental Biology, University of California San Diego, La Jolla, California 92093–0116 (Y.C., Y.Zhao); and State Key LaboraChao ZhouState Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, China (Z.J.C., L.W., W.S., Y.Zhang, C.Z., Y.H.S., W.L., T.T.S., X.Y.Z., X.G.L., X.S.Z.); Section of Cell and Developmental Biology, University of California San Diego, La Jolla, California 92093–0116 (Y.C., Y.Zhao); and State Key LaboraYing Hua SuState Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, China (Z.J.C., L.W., W.S., Y.Zhang, C.Z., Y.H.S., W.L., T.T.S., X.Y.Z., X.G.L., X.S.Z.); Section of Cell and Developmental Biology, University of California San Diego, La Jolla, California 92093–0116 (Y.C., Y.Zhao); and State Key LaboraWei LiState Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, China (Z.J.C., L.W., W.S., Y.Zhang, C.Z., Y.H.S., W.L., T.T.S., X.Y.Z., X.G.L., X.S.Z.); Section of Cell and Developmental Biology, University of California San Diego, La Jolla, California 92093–0116 (Y.C., Y.Zhao); and State Key LaboraTian SunState Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, China (Z.J.C., L.W., W.S., Y.Zhang, C.Z., Y.H.S., W.L., T.T.S., X.Y.Z., X.G.L., X.S.Z.); Section of Cell and Developmental Biology, University of California San Diego, La Jolla, California 92093–0116 (Y.C., Y.Zhao); and State Key LaboraXiang ZhaoState Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, China (Z.J.C., L.W., W.S., Y.Zhang, C.Z., Y.H.S., W.L., T.T.S., X.Y.Z., X.G.L., X.S.Z.); Section of Cell and Developmental Biology, University of California San Diego, La Jolla, California 92093–0116 (Y.C., Y.Zhao); and State Key LaboraXing Guo LiState Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, China (Z.J.C., L.W., W.S., Y.Zhang, C.Z., Y.H.S., W.L., T.T.S., X.Y.Z., X.G.L., X.S.Z.); Section of Cell and Developmental Biology, University of California San Diego, La Jolla, California 92093–0116 (Y.C., Y.Zhao); and State Key LaboraYoufa ChengState Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, China (Z.J.C., L.W., W.S., Y.Zhang, C.Z., Y.H.S., W.L., T.T.S., X.Y.Z., X.G.L., X.S.Z.); Section of Cell and Developmental Biology, University of California San Diego, La Jolla, California 92093–0116 (Y.C., Y.Zhao); and State Key LaboraYunde ZhaoState Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, China (Z.J.C., L.W., W.S., Y.Zhang, C.Z., Y.H.S., W.L., T.T.S., X.Y.Z., X.G.L., X.S.Z.); Section of Cell and Developmental Biology, University of California San Diego, La Jolla, California 92093–0116 (Y.C., Y.Zhao); and State Key LaboraQi XieState Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, China (Z.J.C., L.W., W.S., Y.Zhang, C.Z., Y.H.S., W.L., T.T.S., X.Y.Z., X.G.L., X.S.Z.); Section of Cell and Developmental Biology, University of California San Diego, La Jolla, California 92093–0116 (Y.C., Y.Zhao); and State Key LaboraXian Sheng ZhangState Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, China (Z.J.C., L.W., W.S., Y.Zhang, C.Z., Y.H.S., W.L., T.T.S., X.Y.Z., X.G.L., X.S.Z.); Section of Cell and Developmental Biology, University of California San Diego, La Jolla, California 92093–0116 (Y.C., Y.Zhao); and State Key Labora

2012en

ABI

Аннотация

De novo organ regeneration is an excellent biological system for the study of fundamental questions regarding stem cell initiation, cell fate determination, and hormone signaling. Despite the general belief that auxin and cytokinin responses interact to regulate de novo organ regeneration, the molecular mechanisms underlying such a cross talk are little understood. Here, we show that spatiotemporal biosynthesis and polar transport resulted in local auxin distribution in Arabidopsis (Arabidopsis thaliana), which in turn determined the cytokinin response during de novo shoot regeneration. Genetic and pharmacological interference of auxin distribution disrupted the cytokinin response and ATP/ADP ISOPENTENYLTRANSFERASE5 (AtIPT5) expression, affecting stem cell initiation and meristem formation. Transcriptomic data suggested that AUXIN RESPONSE FACTOR3 (ARF3) mediated the auxin response during de novo organ regeneration. Indeed, mutations in ARF3 caused ectopic cytokinin biosynthesis via the misexpression of AtIPT5, and this disrupted organ regeneration. We further showed that ARF3 directly bound to the promoter of AtIPT5 and negatively regulated AtIPT5 expression. The results from this study thus revealed an auxin-cytokinin cross talk mechanism involving distinct intermediate signaling components required for de novo stem cell initiation and shed new light on the mechanisms of organogenesis in planta.

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Идентификаторы

DOI: 10.1104/pp.112.203166

Цитирования и источники

Цитирований: 2Использованных источников: 0

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