Статья

Shoot organogenesis and somatic embryogenesis from leaf and root explants of Scaevola sericea

Hanzhi LiangCollege of Horticulture and Landscape Architecture, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, ChinaYuping XiongCollege of Horticulture and Landscape Architecture, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, China. [email protected]Beiyi GuoGuangdong Provincial Key Laboratory of Applied Botany and Engineering Laboratory for Vegetation Ecosystem Restoration On Islands and Coastal Zones, South China Botanical Garden, The Chinese Academy of Sciences, Guangzhou, 510650, ChinaHaifeng YanCash Crop Institute of Guangxi Academy of Agricultural Sciences, Nanning, ChinaShuguang JianGuangdong Provincial Key Laboratory of Applied Botany and Engineering Laboratory for Vegetation Ecosystem Restoration On Islands and Coastal Zones, South China Botanical Garden, The Chinese Academy of Sciences, Guangzhou, 510650, ChinaHai RenGuangdong Provincial Key Laboratory of Applied Botany and Engineering Laboratory for Vegetation Ecosystem Restoration On Islands and Coastal Zones, South China Botanical Garden, The Chinese Academy of Sciences, Guangzhou, 510650, ChinaXinhua ZhangGuangdong Provincial Key Laboratory of Applied Botany and Engineering Laboratory for Vegetation Ecosystem Restoration On Islands and Coastal Zones, South China Botanical Garden, The Chinese Academy of Sciences, Guangzhou, 510650, ChinaYuan LiGuangdong Provincial Key Laboratory of Applied Botany and Engineering Laboratory for Vegetation Ecosystem Restoration On Islands and Coastal Zones, South China Botanical Garden, The Chinese Academy of Sciences, Guangzhou, 510650, ChinaSongjun ZengGuangdong Provincial Key Laboratory of Applied Botany and Engineering Laboratory for Vegetation Ecosystem Restoration On Islands and Coastal Zones, South China Botanical Garden, The Chinese Academy of Sciences, Guangzhou, 510650, ChinaKunlin WuGuangdong Provincial Key Laboratory of Applied Botany and Engineering Laboratory for Vegetation Ecosystem Restoration On Islands and Coastal Zones, South China Botanical Garden, The Chinese Academy of Sciences, Guangzhou, 510650, ChinaFeng ZhengGuangdong Provincial Key Laboratory of Applied Botany and Engineering Laboratory for Vegetation Ecosystem Restoration On Islands and Coastal Zones, South China Botanical Garden, The Chinese Academy of Sciences, Guangzhou, 510650, ChinaJaime A. Teixeira da SilvaMiki-cho Post Office, P.O. Box 7, Ikenobe 3011-2, Miki-cho, Kagawa-ken, 761-0799, Japan. [email protected]Youhua XiongCollege of Horticulture and Landscape Architecture, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, China. [email protected]Guohua MaGuangdong Provincial Key Laboratory of Applied Botany and Engineering Laboratory for Vegetation Ecosystem Restoration On Islands and Coastal Zones, South China Botanical Garden, The Chinese Academy of Sciences, Guangzhou, 510650, China. [email protected]

2020en

ABI

Аннотация

An efficient regeneration system via shoot organogenesis and somatic embryogenesis from in vitro leaf and root explants was established for Scaevola sericea for the first time. The highest axillary shoot proliferation coefficient (4.8) was obtained on Murashige and Skoog (MS) medium supplemented with 1.0 mg/L 6-benzyladenine (BA) and 0.1 mg/L α-naphthaleneacetic acid (NAA) every 45 days. Young in vitro leaves and roots, which were used as explants, were cultured onto medium supplemented with different plant growth regulators. Our results showed that only cytokinins BA and thidiazuron (TDZ), could induce adventitious shoots and somatic embryos from leaf and root explants. The optimal medium to achieve this was MS medium supplemented with 2.5 mg/L BA and which induced most adventitious shoots (2.7) and somatic embryos (17.3) from leaf explants within 30 days. From root explants, 1.1 adventitious shoots and 7.6 somatic embryos could be induced on MS medium supplemented with 2.5 mg/L TDZ. Histological observation showed that both somatic embryos and adventitious shoots were originated from homogeneous parenchyma and the development of somatic embryos was visible. Maximum rooting percentage (99.0%) was achieved on half-strength MS medium supplemented with 2.5 mg/L NAA. Well-rooted plantlets, which were transplanted into a substrate of pure river sand, displayed a high survival percentage of 91.7% after transplanting for 45 days while the best substrate for plantlet growth was river sand: coral sand (1:1).

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Идентификаторы

DOI: 10.1038/s41598-020-68084-1

Цитирования и источники

Цитирований: 2Использованных источников: 0

Показатели — AkademScholar