Статья

SlMYB72 Regulates the Metabolism of Chlorophylls, Carotenoids, and Flavonoids in Tomato Fruit

Mengbo WuCenter of Plant Functional Genomics, Institute of Advanced Interdisciplinary Studies, Chongqing University, 401331 Chongqing, ChinaXin XuCenter of Plant Functional Genomics, Institute of Advanced Interdisciplinary Studies, Chongqing University, 401331 Chongqing, ChinaXiaowei HuCenter of Plant Functional Genomics, Institute of Advanced Interdisciplinary Studies, Chongqing University, 401331 Chongqing, ChinaYudong LiuCenter of Plant Functional Genomics, Institute of Advanced Interdisciplinary Studies, Chongqing University, 401331 Chongqing, ChinaHaohao CaoCenter of Plant Functional Genomics, Institute of Advanced Interdisciplinary Studies, Chongqing University, 401331 Chongqing, ChinaHelen M. ChanDepartment of Plant Sciences, University of California, Davis, California 95616Zehao GongCenter of Plant Functional Genomics, Institute of Advanced Interdisciplinary Studies, Chongqing University, 401331 Chongqing, ChinaYujin YuanCenter of Plant Functional Genomics, Institute of Advanced Interdisciplinary Studies, Chongqing University, 401331 Chongqing, ChinaYingqing LuoCenter of Plant Functional Genomics, Institute of Advanced Interdisciplinary Studies, Chongqing University, 401331 Chongqing, ChinaBihong FengCollege of Agriculture, Guangxi University, Nanning 530004, ChinaZhengguo LiCenter of Plant Functional Genomics, Institute of Advanced Interdisciplinary Studies, Chongqing University, 401331 Chongqing, ChinaWei DengCenter of Plant Functional Genomics, Institute of Advanced Interdisciplinary Studies, Chongqing University, 401331 Chongqing, China

2020en

ABI

Аннотация

Tomato (Solanum lycopersicum) fruit ripening is accompanied by the degradation of chlorophylls and the accumulation of carotenoids and flavonoids. Tomato SlMYB72 belongs to the R2R3 MYB subfamily, is located in the nucleus, and possesses transcriptional activator activity. Down-regulation of the SlMYB72 gene produced uneven-colored fruits; that is, dark green spots appeared on immature and mature green fruits, whereas yellow spots appeared on red fruits. Down-regulation of SlMYB72 increased chlorophyll accumulation, chloroplast biogenesis and development, and photosynthesis rate in fruits. This down-regulation decreased lycopene content, promoted b-carotene production and chromoplast development, and increased flavonoid accumulation in fruits. RNA sequencing analysis revealed that down-regulation of SlMYB72 altered the expression levels of genes involved in the biosynthesis of chlorophylls, carotenoids, and flavonoids. SlMYB72 protein interacted with the auxin response factor SlARF4. SlMYB72 directly targeted protochlorophyllide reductase, Mg-chelatase H subunit, and knotted1-like homeobox2 genes and regulated chlorophyll biosynthesis and chloroplast development. SlMYB72 directly bound to phytoene synthase, z-carotene isomerase, and lycopene b-cyclase genes and regulated carotenoid biosynthesis. SlMYB72 directly targeted 4-coumarate-coenzyme A ligase and chalcone synthase genes and regulated the biosynthesis of flavonoids and phenolic acid. The uneven color phenotype in RNA interference-SlMYB72 fruits was due to uneven silencing of SlMYB72 and uneven expression of chlorophyll, carotenoid, and flavonoid biosynthesis genes. In summary, this study identified important roles for SlMYB72 in the regulation of chlorophyll, carotenoid, and flavonoid metabolism and provided a potential target to improve fruit nutrition in horticultural crops.

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Идентификаторы

DOI: 10.1104/pp.20.00156

Цитирования и источники

Цитирований: 2Использованных источников: 0

Показатели — AkademScholar