Статья

Measurement of Cell Migration on Surface-Bound Fibronectin Gradients

Jason T. SmithDepartment of Biomedical Engineering and Department of Biostatistics and Bioinformatics, Duke University, Durham, North Carolina 27708, and Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716John K. TomfohrDepartment of Biomedical Engineering and Department of Biostatistics and Bioinformatics, Duke University, Durham, North Carolina 27708, and Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716Matthew C. WellsDepartment of Biomedical Engineering and Department of Biostatistics and Bioinformatics, Duke University, Durham, North Carolina 27708, and Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716Thomas P. BeebeDepartment of Biomedical Engineering and Department of Biostatistics and Bioinformatics, Duke University, Durham, North Carolina 27708, and Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716Thomas B. KeplerDepartment of Biomedical Engineering and Department of Biostatistics and Bioinformatics, Duke University, Durham, North Carolina 27708, and Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716W. Monty ReichertDepartment of Biomedical Engineering and Department of Biostatistics and Bioinformatics, Duke University, Durham, North Carolina 27708, and Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716

2004en

ABI

Аннотация

A novel technique for the quantitative observation of cell migration along linear gradient substrates functionalized with adhesive proteins is presented. Gradients of the cell adhesion molecule fibronectin are generated by the cross diffusion of functionalizable alkanethiols on gold and characterized by X-ray photoelectron spectroscopy and surface plasmon resonance. Two distinct migration assays are described that characterize the movement of either sparsely populated noncontacting cells or a confluent monolayer of cells into free space. The drift speed of bovine aortic endothelial cells is measured and shown to increase along a fibronectin gradient when compared to a uniform control substrate using both assays. The results of these experiments establish reproducible conditions for studies of cell migration on gradients of surface-bound ligands.

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Идентификаторы

DOI: 10.1021/la0489763

Цитирования и источники

Цитирований: 2Использованных источников: 0

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