Статья

Individuality in gut microbiota composition is a complex polygenic trait shaped by multiple environmental and host genetic factors

Andrew K. BensonDepartment of Food Science and Technology and Core for Applied Genomics and Ecology, University of Nebraska, Lincoln, NE 68583-0919;Scott A. KellyDepartment of Genetics, Carolina Center for Genome Science, University of North Carolina, Chapel Hill, NC 27599-7545;Ryan LeggeDepartment of Food Science and Technology and Core for Applied Genomics and Ecology, University of Nebraska, Lincoln, NE 68583-0919;Fangrui MaDepartment of Food Science and Technology and Core for Applied Genomics and Ecology, University of Nebraska, Lincoln, NE 68583-0919;Soo Jen LowDepartment of Food Science and Technology and Core for Applied Genomics and Ecology, University of Nebraska, Lincoln, NE 68583-0919;Jaehyoung KimDepartment of Food Science and Technology and Core for Applied Genomics and Ecology, University of Nebraska, Lincoln, NE 68583-0919;Min ZhangDepartment of Food Science and Technology and Core for Applied Genomics and Ecology, University of Nebraska, Lincoln, NE 68583-0919;Phaik Lyn OhDepartment of Food Science and Technology and Core for Applied Genomics and Ecology, University of Nebraska, Lincoln, NE 68583-0919;Derrick L. NehrenbergDepartment of Genetics, Carolina Center for Genome Science, University of North Carolina, Chapel Hill, NC 27599-7545;Kunjie HuaDepartment of Genetics, Carolina Center for Genome Science, University of North Carolina, Chapel Hill, NC 27599-7545;Stephen D. KachmanDepartment of Statistics, University of Nebraska, Lincoln, NE 68583-0963;Etsuko N. MoriyamaSchool of Biological Sciences and Center for Plant Science Innovation, University of Nebraska, Lincoln, NE 68588-0118; andJens WalterDepartment of Food Science and Technology and Core for Applied Genomics and Ecology, University of Nebraska, Lincoln, NE 68583-0919;Daniel A. PetersonDepartment of Food Science and Technology and Core for Applied Genomics and Ecology, University of Nebraska, Lincoln, NE 68583-0919;Daniel PompDepartment of Genetics, Carolina Center for Genome Science, University of North Carolina, Chapel Hill, NC 27599-7545;

2010en

ABI

Аннотация

In vertebrates, including humans, individuals harbor gut microbial communities whose species composition and relative proportions of dominant microbial groups are tremendously varied. Although external and stochastic factors clearly contribute to the individuality of the microbiota, the fundamental principles dictating how environmental factors and host genetic factors combine to shape this complex ecosystem are largely unknown and require systematic study. Here we examined factors that affect microbiota composition in a large (n = 645) mouse advanced intercross line originating from a cross between C57BL/6J and an ICR-derived outbred line (HR). Quantitative pyrosequencing of the microbiota defined a core measurable microbiota (CMM) of 64 conserved taxonomic groups that varied quantitatively across most animals in the population. Although some of this variation can be explained by litter and cohort effects, individual host genotype had a measurable contribution. Testing of the CMM abundances for cosegregation with 530 fully informative SNP markers identified 18 host quantitative trait loci (QTL) that show significant or suggestive genome-wide linkage with relative abundances of specific microbial taxa. These QTL affect microbiota composition in three ways; some loci control individual microbial species, some control groups of related taxa, and some have putative pleiotropic effects on groups of distantly related organisms. These data provide clear evidence for the importance of host genetic control in shaping individual microbiome diversity in mammals, a key step toward understanding the factors that govern the assemblages of gut microbiota associated with complex diseases.

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Идентификаторы

DOI: 10.1073/pnas.1007028107

Цитирования и источники

Цитирований: 2Использованных источников: 0

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