Статья

Antimicrobial Activity of Different Artemisia Essential Oil Formulations

Sourav DasDepartment of Laboratory Medicine, University of Pécs, Medical School, 7624 Pécs, Ifjúság u. 13., HungaryBarbara Vörös-HorváthInstitute of Pharmaceutical Technology and Biopharmacy, University of Pécs, Faculty of Pharmacy, Rókus u. 2., 7624 Pécs, HungaryTímea BencsikDepartment of Pharmacognosy, University of Pécs, Faculty of Pharmacy, Rókus u. 2., 7624 Pécs, HungaryGiuseppe MicalizziChromaleont s.r.l., c/o Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98168 Messina, ItalyLuigi MondelloChromaleont s.r.l., c/o Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98168 Messina, ItalyGyörgyi HorváthDepartment of Pharmacognosy, University of Pécs, Faculty of Pharmacy, Rókus u. 2., 7624 Pécs, HungaryTamás KőszegiDepartment of Laboratory Medicine, University of Pécs, Medical School, 7624 Pécs, Ifjúság u. 13., HungaryAleksandar SzéchenyiInstitute of Pharmaceutical Technology and Biopharmacy, University of Pécs, Faculty of Pharmacy, Rókus u. 2., 7624 Pécs, Hungary

2020en

ABI

Аннотация

The extreme lipophilicity of essential oils (EOs) impedes the measurement of their biological actions in an aqueous environment. We formulated oil in water type Pickering Artemisia annua EO nanoemulsions (AEP) with surface-modified Stöber silica nanoparticles (20 nm) as the stabilizing agent. The antimicrobial activity of AEP and its effects on mature Candida biofilms were compared with those of Tween 80 stabilized emulsion (AET) and ethanolic solution (AEE) of the Artemisia EO. The antimicrobial activity was evaluated by using the minimum inhibitory concentrations (MIC90) and minimum effective concentrations (MEC10) of the compounds. On planktonic bacterial and fungal cells beside growth inhibition, colony formation (CFU/mL), metabolic activity, viability, intracellular ATP/total protein (ATP/TP), along with reactive oxygen species (ROS) were also studied. Artemisia annua EO nanoemulsion (AEP) showed significantly higher antimicrobial activity than AET and AEE. Artemisia annua EO nanoemulsions (AEP) generated superoxide anion and peroxides-related oxidative stress, which might be the underlying mode of action of the Artemisia EO. Unilamellar liposomes, as a cellular model, were used to examine the delivery efficacy of the EO of our tested formulations. We could demonstrate higher effectiveness of AEP in the EO components’ donation compared to AET and AEE. Our data suggest the superiority of the AEP formulation against microbial infections.

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Идентификаторы

DOI: 10.3390/molecules25102390

Цитирования и источники

Цитирований: 2Использованных источников: 0

Показатели — AkademScholar