Marker-assisted selection to improve submergence tolerance, blast resistance and strong fragrance in glutinous rice
Аннотация
Glutinous rice is popularly grown for consumption in Lao PDR. Most of the Laotian glutinous rice varieties grew nowadays are intolerant to flash flooding, susceptible to major diseases such as blast and bacterial blight and lacking of good grain and cooking quality. Our goal of this study is to develop new glutinous rice varieties that have good cooking quality, tolerance to flash flooding and resistance to blast disease by combining Sub1 , badh2 , qBl1 and qBl11 loci from the glutinous elite lines IR85264 ( Sub1 ), TDK303 ( badh2 ) and RGD07529 ( qBl1 + qBl11 ) through marker-assisted foreground selection (MAS) strategy. Three-way crossing was made to develop a breeding population in which MAS was applied in the series to select for individuals carrying submergence tolerance ( Sub1 ), blast resistance ( qBl1 , qBl11 ) and fragrance ( badh2 ). According to the marker genotypes, twenty eight F 5 homozygous lines carrying Sub1 , badh2 , qBl1 and qBl11 loci were finally selected and evaluated for submergence tolerance (SUBT), blast resistance (BLR) and fragrance (FR). All F 5 selected lines are fragrance and showed high level of SUBT and BLR. This study provides further support on the effective of MAS to improve FR, SUBT and BLR in rice breeding program.