In vitro propagation of Acacia mangium × A. auriculiformis

【Objective】To improve the propagation efficiency and extend the planting of Acacia mangium ×A. auriculiformis.【Method】This study was carried out to explore the techniques of in vitro propagation of A. mangium × A. auriculiformis using stem segments with buds collected from 16-year plants as the explant. 【Result and conclusion】Current season stem segments carrying 3-5 axillary buds were sterilized with w = 0. 1% mercuric chloride and φ = 75% alcoho1 for 15 min and 30 s respectively before they were inoculated onto MS medium. Buds could be induced successfully on MS with a shooting rate of 95. 68%.The bud proliferation index was 3. 97 after being transferred onto MS + 6-BA 1. 5 mg·L-1+ NAA 0. 1mg·L-1+ sucrose 30-40 g·L-1for 35 d. The buds all rooted on the 15 thday from inoculation onto1 /2 MS medium after pre-cultured on MS supplemented with IBA 600 mg·L-1for 4-8 h. Inoculating the proliferated buds onto 1 /2 MS + IBA 1. 0 mg·L-1+ NAA 0. 5 mg·L-1+ sucrose 30 g·L-1also generated a high rooting rate of 99. 43%. The survival rate reach 94. 67% after the rooted plantlet are transplanted to the yellow soil medium.

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