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Genome Editing in Plants: An Overview of Tools and Applications

Venera KamburovaCenter of Genomics and Bioinformatics, Academy of Sciences of the Republic of Uzbekistan, University Street-2, Qibray Region, 111215 Tashkent, UzbekistanElena NikitinaCenter of Genomics and Bioinformatics, Academy of Sciences of the Republic of Uzbekistan, University Street-2, Qibray Region, 111215 Tashkent, UzbekistanShukhrat E. ShermatovCenter of Genomics and Bioinformatics, Academy of Sciences of the Republic of Uzbekistan, University Street-2, Qibray Region, 111215 Tashkent, UzbekistanZabardast T. BurievCenter of Genomics and Bioinformatics, Academy of Sciences of the Republic of Uzbekistan, University Street-2, Qibray Region, 111215 Tashkent, UzbekistanSiva P. KumpatlaChandrakanth EmaniWestern Kentucky University-Owensboro, Owensboro, KY 42303, USAIbrokhim Y. AbdurakhmonovCenter of Genomics and Bioinformatics, Academy of Sciences of the Republic of Uzbekistan, University Street-2, Qibray Region, 111215 Tashkent, Uzbekistan
ABI

Аннотация

The emergence of genome manipulation methods promises a real revolution in biotechnology and genetic engineering. Targeted editing of the genomes of living organisms not only permits investigations into the understanding of the fundamental basis of biological systems but also allows addressing a wide range of goals towards improving productivity and quality of crops. This includes the creation of plants with valuable compositional properties and with traits that confer resistance to various biotic and abiotic stresses. During the past few years, several novel genome editing systems have been developed; these include zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and clustered regularly interspaced short palindromic repeats/Cas9 (CRISPR/Cas9). These exciting new methods, briefly reviewed herein, have proved themselves as effective and reliable tools for the genetic improvement of plants.

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