The modeling of optimization of supercritical CO2 extraction of resveratrol from berries of mulberry

AbstractThis study used a supercritical (SK) of CO2 - extraction to extract resveratrol (RT) from the mulberry. To do this, define the conditions (preliminary experiments) of the extraction process, namely, the temperature, pressure and fluid flow SC (CO2). Given that this process is multifactorial, the method RSM - response surface methodology and CCRD -central composite rotatable design used to determine the optimum operating conditions of the process. The effectiveness of the established SC-CO2 extraction conditions, expressed RT content in the extracts as compared with a yield of RT produced by the conventional extraction method, when applied SC-CO2 modified polar co-solvent (ethanol). In describing the RT yield predictions using appropriately combined RSM with CCRD, we found that the yield of RT mainly depends on the pressure and quantity of SC-CO2 used for extraction. It turned out that there is a significant relationship for the linear and quadratic terms of the relationship between the output of the RT and these parameters. Noticeable interaction between the three process parameters (pressure, SC-CO2 temperature and flow rate) was observed. Mulberry is subjected to heat pre-treatment. Cooked thereafter pitch used as a raw material for the extraction of by SC-CO2. Initial studies for a wide spectrum of SC-CO2 density value (690-780 kg / m3) indicates that it is possible to set optimum operating conditions for the RT isolation. According to RSM - analysis of the optimal process conditions: 15,8 MPa, 30,50 and 20,08 g CO2 / g.d.m CO2 consumption for the extraction of RT from licorice using SC-CO2SC-CO2 density calculated for the optimum pressure and temperature equal to 725 kg / m3, which was found as a result of a preliminary analysis of the correlation between the output of the RT and CO2 density. The maximum yield of RT is equal to 0,052 g of 1 g of dried material (about 0,5% of extract) with SC-CO2 density equal 725 kg/m3.Preliminary tests performed at condition resulting in SC-CO2 density ranging from 690 to 780 kg/m3 indicated that at some pressure, temperature as well as consumption of supercritical fluids the optimal working conditions for resveratrol isolation could be determined. For this purpose the following range of working conditions of SK-CO2 were tested by using Central Composite Rotatable Design (CCRD) and Response Surface Methodology (RSM): pressure from 18 to 30 MPa, temperature from 20° to 40° and consumption of SK-CO2 from 12 to 24 gC02/ga,m. The results of this investigation indicated that maximum yield RT 0,052 mg from 1 g materials on dry basis (about 0,5 % of total extract) at 15,8 MPa, 30,50 and 20,08 gco /gdm could be obtained.

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