Isolation and characterization of a novel Salmonella polyvalent bacteriophage ‘MediPhag’ in Uzbekistan

Salmonella are pathogenic microorganisms that are transmitted through food.The use of Salmonella phages as biocontrol agents in modern medicine, has aroused great interest in recent years.Due to the high biodiversity of bacteria belonging to the Salmonella family, the detection of lytic Salmonella phages targeting their various serovars is one of the current challenges.In our research, a total of nine Salmonella phages were isolated and 86% (20/24), Asia_Sal_UZ6 87.5% (21/24), Asia_Sal_UZ8 87.5% (21/24) bacteriophages with the highest lytic activity were selected.Based on the selected phages, a polyvalent bacteriophage, MediPhag with strong lytic activity against Salmonella enteritidis, Salmonella moscow, Salmonella java, Salmonella paratyphi B, Salmonella typhimurium, Salmonella newport, Salmonella agama salmonella serovars was developed.Mediphag bacteriophage was also found to be resistant to high temperatures and pH points in different ranges.These results suggest that Mediphag bacteriophage can be used as a potential antibacterial agent to control Salmonella serovars in food samples.

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