PS-C30-3: FEATURES OF THE C-786T NOS3 GENE POLYMORPHISM IN PREGNANT WOMEN WITH PREECLAMPSIA

Relevance: Preeclampsia is the multi cause illness. One of the genetic risk factors for the development of preeclampsia may be a polymorphism of the endothelial system genes. Objective: to study the C-786T polymorphism of the NOS3 gene in patients with preeclampsia. Materials and methods: The study group consisted of 140 pregnant women, of which the main group included 72 patients with preeclampsia, and 68 apparently healthy individuals (control group). The study was conducted in the Tashkent city maternity complex 6. The age of the pregnant women ranged from 19 to 42 years. The material for the study was DNA samples from a patient with a clinically diagnosed preeclampsia. Statistical processing of the results was carried out using statistical programs “EpiCalc 2000 Version 1.02“. Results: The proportion of wild C allele in the subgroup of patients was statistically significantly lower compared to the control (68.6% versus 79.9%, respectively; (p = 0.04; RR = 0.86; OR = 0.55; 95%). On the contrary, the frequency of the unfavorable T allele among patients with severe preeclampsia was significantly higher compared to controls - 31.4% and 20.1%, respectively (p = 0.04). At the same time, the calculated odds ratio for detecting this allele was OR = 1.8, and the relative risk of developing diseases was RR = 1.17 (p = 0.04). There was a trend towards an increase in the heterozygous C/T genotype in the group of patients with severe preeclampsia, where it was detected with a frequency of 39.5% compared to 27.1% in the control group (p = 0.15; RR = 1.46; OR = 1.76). The functionally unfavorable T/T genotype was insignificantly more common among patients with severe preeclampsia compared to the control group 11.6% and 6.5%, respectively (p = 0.30; RR = 1.78; OR = 1.88;). A significant relationship was found between the risk of developing preeclampsia and the carriage of a rare allelic variant of the C-786T polymorphism of the NOS3 gene, associated with a decrease in the synthesis of the NO-synthetase enzyme. Conclusions: Thus, the results of our study allow us to conclude that unfavorable genotypic variants of the C-786T polymorphism of the NOS3 gene (associated with vascular endothelial dysfunction) contribute significantly to the mechanism of preeclampsia development.

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