Мақола

Gut Bacteria Mediate Aggregation Pheromone Release in the Borer Beetle Trigonorhinus sp.

Jinyang DongDepartment of Forest Conservation, Forestry College, Inner Mongolia Agricultural University, Hohhot 010018, ChinaXiang YaoDepartment of Forest Conservation, Forestry College, Inner Mongolia Agricultural University, Hohhot 010018, ChinaYanru ZhangDepartment of Forest Conservation, Forestry College, Inner Mongolia Agricultural University, Hohhot 010018, ChinaXiuhua WuXinhai LiuUlanqab Institute of Agricultural and Forestry Sciences, Ulanqab 012001, ChinaHongbin ZhangUlanqab Forestry Protection Station, Ulanqab 012000, ChinaHaiyan JiangDepartment of Forest Conservation, Forestry College, Inner Mongolia Agricultural University, Hohhot 010018, ChinaJianli HouUlanqab Forestry Protection Station, Ulanqab 012000, ChinaJie YanUlanqab Institute of Agricultural and Forestry Sciences, Ulanqab 012001, ChinaJianing SunDepartment of Forest Conservation, Forestry College, Inner Mongolia Agricultural University, Hohhot 010018, China

Insectsjournal2025en

ABI

Аннотация

Gut microbial symbionts are increasingly recognized as key modulators of host insect physiology and behavior, yet their role in pheromone-mediated chemical communication remains insufficiently understood. In this study, we investigated the wood-boring beetle Trigonorhinus sp., a pest of Caragana liouana, to determine the necessity of gut bacteria for male aggregation pheromone release. A combination of antibiotic-mediated bacterial depletion, quantitative PCR, gas chromatography-mass spectrometry (GC-MS), and Y-tube olfactometry was employed. Antibiotic treatment resulted in a marked reduction in gut bacterial load and a concomitant decrease of more than 85% in the emission of two key pheromone components, 2,6,10,14-tetramethylheptadecane and heptacosane. Behavioral assays demonstrated that females no longer exhibited significant attraction to treated males. Furthermore, defined recolonization with a single cultured gut isolate, Acinetobacter guillouiae, was sufficient to rescue pheromone emission. This indicates that particular gut taxa, rather than microbial biomass alone, are essential for pheromone biosynthesis. These findings demonstrate a decisive role of gut bacteria in the chemical communication of Trigonorhinus sp. and highlight the potential of symbiont-targeted strategies for pest management.

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Мавзулар

Insect symbiosis and bacterial influences Insect Utilization and Effects Insect and Arachnid Ecology and Behavior

Идентификаторлар

DOI: 10.3390/insects16100999

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