Мақола

Molecular screening of Septoria tritici blotch resistance genes in European bread wheat cultivars using validated gene-specific SSR markers

Rada ŠućurPanCrop Lab, Faculty of Agriculture, University of Novi Sad, Novi Sad, 21000, SerbiaAmjad AliDepartment of Plant Protection, Faculty of Agricultural Sciences and Technologies, Sivas University of Science and Technology, 58000, Sivas, TurkeyVelimir MladenovPanCrop Lab, Faculty of Agriculture, University of Novi Sad, Novi Sad, 21000, SerbiaBojan JockovićInstitute of Field and Vegetable Crops, Novi Sad, 21000, SerbiaJiqing GouFrontiers Science Center for Molecular Design Breeding, China Agricultural University, Beijing, 100193, ChinaMuhammad Azhar NadeemDepartment of Biotechnology, Faculty of Science, Mersin University, 33343, Yenişehir, Mersin, TurkeyAllah BakhshCentre of Excellence in Molecular Biology, University of the Punjab, Lahore, 53700, PakistanRaheela RehmanDepartment of Plant Breeding and Genetics/Centre for Advanced Studies in Agriculture and Food Security (CAS-AFS), University of Agriculture, Faisalabad, 38000, PakistanJazib JavedFrontiers Science Center for Molecular Design Breeding, China Agricultural University, Beijing, 100193, ChinaHÜSNÜ AKTAŞMardin Artuklu University Vocational Higher School of Kzltepe, 47060, Mardin, TurkeyJi MaFrontiers Science Center for Molecular Design Breeding, China Agricultural University, Beijing, 100193, ChinaManh CuongCollege of Medicine and Pharmacy, Duy Tan University, Da Nang, 550000, VietnamFaheem Shehzad BalochDepartment of Biotechnology, Faculty of Science, Mersin University, 33343, Yenişehir, Mersin, Turkey

Phytopathology Researchjournal2026en

ABI

Аннотация

Abstract Zymoseptoria tritici , the causal agent of Septoria tritici blotch (STB), poses a major threat to wheat production in temperate regions worldwide. This study aimed to determine the distribution of 15 known Stb resistance genes in 60 bread wheat cultivars from Serbia, Croatia, France, Italy, Romania, Hungary, and Mexico using validated gene-specific simple sequence repeat (SSR) markers. Genomic DNA was extracted from 35-day-old seedlings and subjected to PCR amplification using 15 Stb -linked SSR markers. The presence of target alleles was confirmed via 2% agarose gel electrophoresis. Results showed that Stb1 was present in all genotypes (60/60 = 100%), highlighting its widespread use. The 44.1% (60/136) gene detection rate was recorded for Stb1 ; in contrast, Stb9 and Stb16 were not amplified in any sample, suggesting their absence within the evaluated germplasm. Other frequently detected genes included Stb12 (33.3%), Stb14 (23.3%), and Stb4 (15%). Genes like Stb2 , Stb3 , and Stb6 were identified in only 5%, 5%, and 3.3% of genotypes, respectively. The French cultivar ‘Falado’ possessed the highest number of resistance genes (7), followed by ‘Cellule’, ‘Solindo’, ‘Sofolk’, and Serbian cultivars ‘NS Epoha’ and ‘NS Grivna’ with five genes each. These results support gene pyramiding as a strategy for durable resistance. In conclusion, while Stb1 remains predominant, the lack of diversity increases vulnerability to resistance breakdown. Underutilized genes such as Stb6 , Stb8 , and Stb17 offer potential for future resistance breeding. The study emphasizes integrating SSR-based screening with advanced tools like GWAS and functional genomics to enhance marker-assisted selection and promote sustainable wheat improvement.

Ҳали таржима қилинмаган

Мавзулар

Wheat and Barley Genetics and Pathology Mycotoxins in Agriculture and Food Fungal and yeast genetics research

Идентификаторлар

DOI: 10.1186/s42483-026-00411-2

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