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RNAi-Mediated Silencing of vATPase Subunit E Impairs Larval Development in Plutella xylostella, and Virtual Screening Identifies a Potential Inhibitor

Xuetao YuKey Laboratory of Agricultural Genetically Modified Organisms Traceability, Yangluo Station of Chinese Agrosystem Long-Term Observation Network, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Ministry of Agriculture and Rural Affairs, Wuhan 430062, ChinaJinhua LuoEnshi Tujia and Miao Autonomous Prefecture Academy of Agricultural Sciences, Enshi 445000, ChinaJiayi XueKey Laboratory of Agricultural Genetically Modified Organisms Traceability, Yangluo Station of Chinese Agrosystem Long-Term Observation Network, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Ministry of Agriculture and Rural Affairs, Wuhan 430062, ChinaLin LuKey Laboratory of Agricultural Genetically Modified Organisms Traceability, Yangluo Station of Chinese Agrosystem Long-Term Observation Network, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Ministry of Agriculture and Rural Affairs, Wuhan 430062, ChinaPan DengInstitute of Leisure Agriculture, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaLi ZhuKey Laboratory of Agricultural Genetically Modified Organisms Traceability, Yangluo Station of Chinese Agrosystem Long-Term Observation Network, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Ministry of Agriculture and Rural Affairs, Wuhan 430062, ChinaKang YangKey Laboratory of Agricultural Genetically Modified Organisms Traceability, Yangluo Station of Chinese Agrosystem Long-Term Observation Network, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Ministry of Agriculture and Rural Affairs, Wuhan 430062, ChinaXia WanKey Laboratory of Agricultural Genetically Modified Organisms Traceability, Yangluo Station of Chinese Agrosystem Long-Term Observation Network, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Ministry of Agriculture and Rural Affairs, Wuhan 430062, ChinaYuhua WuKey Laboratory of Agricultural Genetically Modified Organisms Traceability, Yangluo Station of Chinese Agrosystem Long-Term Observation Network, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Ministry of Agriculture and Rural Affairs, Wuhan 430062, ChinaAkmal BoboevEnology & Technology of Fermentation Products, Tashkent Institute of Chemical Technology, 32, Navoi Str, Tashkent 100011, UzbekistanGang WuKey Laboratory of Agricultural Genetically Modified Organisms Traceability, Yangluo Station of Chinese Agrosystem Long-Term Observation Network, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Ministry of Agriculture and Rural Affairs, Wuhan 430062, ChinaXiaohong YanKey Laboratory of Agricultural Genetically Modified Organisms Traceability, Yangluo Station of Chinese Agrosystem Long-Term Observation Network, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Ministry of Agriculture and Rural Affairs, Wuhan 430062, ChinaChen‐Hui ShenKey Laboratory of Agricultural Genetically Modified Organisms Traceability, Yangluo Station of Chinese Agrosystem Long-Term Observation Network, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Ministry of Agriculture and Rural Affairs, Wuhan 430062, China

Insectsjournal2026en

ABI

Аннотация

The diamondback moth (Plutella xylostella) is a devastating global pest of cruciferous crops. This study explores the potential of targeting the vacuolar ATPase subunit E (PxvATPaseE) for its control. We demonstrate that PxvATPaseE is essential for larval development, showing high expression levels in the midgut. RNA interference (RNAi)-mediated silencing of PxvATPaseE resulted in severe growth retardation and dose-dependent mortality, with higher dsRNA doses inducing more sustained effects. Furthermore, computational virtual screening of natural compound libraries identified a high-affinity binder of PxvATPaseE, such as periplocoside D. Our results demonstrate that PxvATPaseE is a promising molecular target for controlling P. xylostella, supporting a dual-strategy approach combining RNAi and targeted chemical inhibition for future pest management solutions.

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DOI: 10.3390/insects17040439

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