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In situ and genetic characterization of Gossypium barbadense populations from the states of Pará and Amapá, Brazil

Vanessa Cavalcante de AlmeidaEmbrapa Algodão, BrazilLúcia Vieira HoffmannEmbrapa Algodão, BrazilGilberto Ken It YokomizoEmbrapa Amapá, BrazilJ. N. da CostaEmbrapa Algodão, BrazilMarc GibandCentre de Coopération Internationale en Recherche Agronomique, FrancePaulo Augusto Vianna BarrosoEmbrapa Algodão, Brazil
2009en
ABI

Аннотация

The objective of this work was to characterize the populations of Gossypium barbadense in the states of Amapá and Pará, Brazil. In situ characterization was conducted through interviews with the owners of the plants and environmental observations. Leaf or petal tissue as well as seed samples were collected for genetic characterization by single sequence repeats markers and for storage in germplasm banks, respectively. The plants were maintained in dooryards and used mainly for medical purposes. The genetic analysis showed no heterozygous plants at the loci tested (f = 1), indicating that reproduction occurs mainly through selfing. The total genetic diversity was high (He = 0.39); and a high level of differentiation was observed between cotton plants from the two states (F ST = 0.36). Conventional methods of in situ maintenance of G. barbadense populations are not applicable. The conservation of the genetic variability of populations present in the two states could be achieved through germplasm collection and establishing of ex situ seed banks.

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