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BUSCO: assessing genome assembly and annotation completeness with single-copy orthologs

Felipe A. SimãoDepartment of Genetic Medicine and Development, University of Geneva Medical School and Swiss Institute of Bioinformatics, rue Michel-Servet 1, 1211 Geneva, SwitzerlandRobert M. WaterhouseDepartment of Genetic Medicine and Development, University of Geneva Medical School and Swiss Institute of Bioinformatics, rue Michel-Servet 1, 1211 Geneva, SwitzerlandPanagiotis IoannidisDepartment of Genetic Medicine and Development, University of Geneva Medical School and Swiss Institute of Bioinformatics, rue Michel-Servet 1, 1211 Geneva, SwitzerlandEvgenia V. KriventsevaDepartment of Genetic Medicine and Development, University of Geneva Medical School and Swiss Institute of Bioinformatics, rue Michel-Servet 1, 1211 Geneva, SwitzerlandEvgeny M. ZdobnovDepartment of Genetic Medicine and Development, University of Geneva Medical School and Swiss Institute of Bioinformatics, rue Michel-Servet 1, 1211 Geneva, Switzerland

2015en

ABI

Аннотация

MOTIVATION: Genomics has revolutionized biological research, but quality assessment of the resulting assembled sequences is complicated and remains mostly limited to technical measures like N50. RESULTS: We propose a measure for quantitative assessment of genome assembly and annotation completeness based on evolutionarily informed expectations of gene content. We implemented the assessment procedure in open-source software, with sets of Benchmarking Universal Single-Copy Orthologs, named BUSCO. AVAILABILITY AND IMPLEMENTATION: Software implemented in Python and datasets available for download from http://busco.ezlab.org. CONTACT: [email protected] SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.

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DOI: 10.1093/bioinformatics/btv351

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