Localization of proteins forming the outer surface of isolated metaphase chromosomes
N. D. BelyaevVladimir G. BudkerVeronika DubrovskayaAndrej A. KimInstitute of Nuclear Physics, Academy of Sciences of Uzbek SSR, Taschkent, settlement Ulugbek, 702132, USSRElena V. KiselevaNovosibirsk Institute of Cytology and Genetics, Siberian Division of the USSR Academy of Sciences, Novosibirsk 630090, USSRSidorov VnInstitute of Nuclear Physics
1992en
Ochiq kirishBronza
ABI
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The outer surface of isolated metaphase chromosomes has been investigated by a method of thermally activated tritium labelling. We show that both chromosomal proteins and DNA are tritium-labelled. Fractionation of the chromosomal proteins reveals that scaffold proteins are the most labelled in condensed and EDTA-decondensed chromosomes. Exposition of some scaffold proteins on the outer surface of metaphase chromosomes is suggested.
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