Population genetic diversity of wild Lycium ruthenicum in Qaidam inferred from AFLP markers
WANG Jin-NanKey Laboratory of Agricultural Ecology and Environment, College of Forestry, Shandong Agricultural University, Tai'an, Shandong 271018, China;CHEN Jin-FuForestry Research Institute of Qinghai Province , Xining 810016 , China; andChen Wu-ShengKey Laboratory of Agricultural Ecology and Environment , College of Forestry , Shandong Agricultural University , Tai'an , Shandong 271018 , China;Zhou Xin-YangSchool of Pharmaceutical Sciences, Shandong University , Jinan 250012, ChinaXu DongKey Laboratory of Agricultural Ecology and Environment , College of Forestry , Shandong Agricultural University , Tai'an , Shandong 271018 , China;Jihong LiKey Laboratory of Agricultural Ecology and Environment, College of Forestry, Shandong Agricultural University, Tai'an, Shandong 271018, China;and QI Xiao
2015en
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亓 晓 1 1 山东农业大学林学院农业生态与环境重点实验室, 山东泰安 271018; 2 青海省林业科学研究所, 西宁 810016 ; 3 山东大学药学院, 济南 250012 摘 要 采用扩增片段长度多态性(AFLP)分子标记技术对青海省柴达木地区5个野生黑果枸杞(Lycium ruthenicum)种群的120 份样品的遗传多样性进行分析。结果表明: 柴达木地区野生黑果枸杞具有很高的遗传多样性, 9对选扩引物共得到1 691条清 晰条带, 其中多态性条带1 678条, 多态性变异率为99.23%,种群间的有效等位基因数为1.471 2, Nei's基因多样性为0.3245, Shannon信息指数为0.436 7。分子方差分析(AMOVA)结果表明: 柴达木地区5个黑果枸杞种群的遗传变异主要存在于种群内 部(92%), 种群间的遗传分化较小(8%, 遗传分化系数0.08)。黑果枸杞种群间的遗传相似系数介于0.970 9-0.992 2之间, 平均 值为0.983 5。种群间的聚类及Mantel检验(γ = 0.336 8, p = 0.806 4)均表明柴达木地区黑果枸杞种群地理距离与遗传距离之间
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