Нарушения окислительного фосфорилирования в модели хронической энтеральной недостаточности и их коррекция

Aim.To study the state of oxidative phosphorylation in a model of chronic enteral insufficiency in the growing rats and to assess the effectiveness of ecdystene in their correction. Materials and methods. Laboratory white mongrel rats were used in the experiments. The animals were divided into 3 groups. 1 group - healthy animals (control). Group 2 - animals on a diet poor in protein (chronic malnutrition), group 3 - animals that received as a corrective drug ecdysthene. Mitochondria were isolated from the liver of 40-day-old rats by differential centrifugation. The parameters of oxidative phosphorylation, respiration rate of isolated mitochondria in the metabolic state V4 and V3, as well as the values of respiratory control and ADP / O were determined. Results. Treatment of rats with protein deficiency for 10 days with the drug ecdystene resulted in a significant increase in the body weight of the rats - an average of 45.5% - and by the 10th day of treatment this value was 71% of the values in the control group (P <0.01). In the group of animals that were on a diet poor in proteins, the oxidative phosphorylation parameters were significantly reduced. The rate of oxygen consumption by the mitochondrial suspension in the functional state of V3 and V4 in sick animals was almost half that in control animals. Respiratory control, in the group of sick animals was reduced by 20%. The ratio of ADP / O was also reduced and was 2.5 vs 2.8 in the control group. When using a corrective ecdystene preparation as a quality, the V3 state was 93.72, V4 was 15.62, and Vdnf was 109.34 ng at O / min / mg protein (P <0.001). The value of the respiratory control was also close to the control and was 6, and the ratio of ADP / O even slightly exceeded the control value and was 3.03 (P <0.05). Сonclusion. We detected a pronounced disconnection of the respiratory chain in the mitochondrial suspension when the animals were kept on a diet poor in proteins and also had a bright corrective effect in the ecdystene. The therapeutic effect of ecdysten revealed its pronounced ability to correct manifestations of chronic enteric insufficiency in growing rats.

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