QUANTITATIVE DETERMINATION OF ARTEANNUIN B – A SESQUITERPENE LACTONE FROM ARTEMISIA ANNUA

The HPLC (High performance liquid chromatography) method for the determination of arteannuin B in the herb Artemisia annua has been developed. The method is suitable for its validation characteristics, such as specificity parameters (convergence of the retention times of artianuin B in the standard and sample), suitability of the chromatographic system (separation efficiency), linearity (R2 = 0.99929 at y = 9.38x + 245.98), range of application (from 50 to 150%), accuracy in terms of repeatability (RSD = 3.45%) and correctness (Z = 100.36 ± 0.56%), determined experimentally, are within the recommended values. As a marker compound, it was proposed to use a standard sample of arteannuin B. For this, a standard sample of arteannuin B was obtained with a purity of at least 99%, which is confirmed IR, 1HNMR, 13CNMR spectral data. Organoleptic and physicochemical parameters were determined. It has been established that the presence of impurities in an amount of not more than 1% is allowed in a standard sample of arteannuin B. It was established that the content of arteannuin B in the raw material should be at least 0.2%. The development and validation HPLC-UV method for the determination of arteannuin B have to used to standardization the herb of Artemisia annua.

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