Expresión de la proteína recombinante PreS2-S del antígeno de superficie del virus de la hepatitis B en Pichia pastoris
С. А. СасмаковYunusov Institute of the Chemistry of Plant Substances , M. Ulugbek Str. 77 , 100170 , Tashkent , UzbekistanOybek N. AshirovYunusov Institute of the Chemistry of Plant Substances , M. Ulugbek Str. 77 , 100170 , Tashkent , UzbekistanJaloliddin AbdurakhmanovYunusov Institute of the Chemistry of Plant Substances , M. Ulugbek Str. 77 , 100170 , Tashkent , UzbekistanShukhrat Sh. KhasanovYunusov Institute of the Chemistry of Plant Substances , M. Ulugbek Str. 77 , 100170 , Tashkent , UzbekistanFarkhod EshboevYunusov Institute of the Chemistry of Plant Substances , M. Ulugbek Str. 77 , 100170 , Tashkent , UzbekistanElvira YusupovaYunusov Institute of the Chemistry of Plant Substances , M. Ulugbek Str. 77 , 100170 , Tashkent , UzbekistanG. PiyakinaYunusov Institute of the Chemistry of Plant Substances , M. Ulugbek Str. 77 , 100170 , Tashkent , UzbekistanShakhnoza S. AzimovaYunusov Institute of the Chemistry of Plant Substances , M. Ulugbek Str. 77 , 100170 , Tashkent , Uzbekistan
2021es
Ochiq kirishOlmos
Litsenziya: cc-by-nc
ABI
Annotatsiya
The aim of this work is the expression of the PreS2-S region of surface antigen of hepatitis B virus (HBV) in yeast Pichia pastoris. A cDNA fragment encoding the Pres2-S protein of HBV was cloned to yeast transfer vectors. Based on cloned new plasmids pPIC3.5-PreS2-S (8707 bp) and pPIC9-PreS2-S (8980 bp) the recombinant strains of P. pastoris producing the PreS2-S region of surface antigen of HBV were obtained. The PAGE electrophoresis and immunoblotting of obtained recombinant PreS2-S protein confirm the molecular weight (34 kDa) and high specificity to the HBV antibodies.
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