AJUGA TURKESTANICA ALTERNATIVE SOURCE FOR EKDISTEN PRODUCTION

The process of ecdysteron extraction from the aerial part of Ajuga turkestanica was studied. On the basis research results established the following extraction conditions: extractant – 95% ethyl alcohol, the particle size of the raw material is 2–5 mm and the process temperature at 20–30 °C. Proposed fivefold ecdysteron extraction from the aerial part of Ajuga turkestanica. To remove hydrophobic impurities from the concentrated and water-diluted extract, proposed was a sequential treatment with extraction benzine and ethyl acetate. From the purified aqueous solution, ecdysteron is extracted with n-butanol. From a concentrated butanol solution, precipitation is recommended of iridoids from acetone. For chromatographic purification, the simultaneous use of aluminum oxide and silica gel in the ratio of the sum of ecdysteroids – sorbent 1 : 20. The quality as eluent mix selected propanol-2 – chloroform in a volumetric ratio of 1 : 3, and for crystallization a mixture of methanol with ethyl acetate in a volume ratio of 1 : 9. The technology has been developed for obtaining the ecdisten substance from the arial part of Ajuga turkestanica.

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