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The yellowhorn MYB transcription factor MYB30 is required for wax accumulation and drought tolerance

Xiaojuan LiuState Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, ChinaZhuo BanState Key Laboratory of Tree Genetics and Breeding , Research Institute of Forestry, , Xiangshan Road, Beijing 100091,Yingying YangState Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, ChinaHuihui XuState Key Laboratory of Tree Genetics and Breeding , Research Institute of Forestry, , Xiangshan Road, Beijing 100091,Yifan CuiState Key Laboratory of Tree Genetics and Breeding , Research Institute of Forestry, , Xiangshan Road, Beijing 100091,Chenxue WangState Key Laboratory of Tree Genetics and Breeding , Research Institute of Forestry, , Xiangshan Road, Beijing 100091,Quanxin BiState Key Laboratory of Tree Genetics and Breeding , Research Institute of Forestry, , Xiangshan Road, Beijing 100091,Haiyan YuChinese Academy of Forestry , Research Institute of Forestry, , Xiangshan Road, Beijing 100091,Libing WangState Key Laboratory of Tree Genetics and Breeding , Research Institute of Forestry, , Xiangshan Road, Beijing 100091,

Tree Physiologyjournal2024en

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Annotatsiya

Yellowhorn (Xanthoceras sorbifolium Bunge) is an economically important tree species in northern China, mainly distributed in arid and semi-arid areas where water resources are scarce. Drought affects its yield and the expansion of its suitable growth area. It was found that the wax content in yellowhorn leaves varied significantly among different germplasms, which had a strong correlation with the drought resistance of yellowhorn. In this study, XsMYB30 was isolated from 'Zhongshi 4' of yellowhorn, a new highly waxy variety. DAP-Seq technology revealed that the pathways associated with fatty acids were significantly enriched in the target genes of XsMYB30. Moreover, the results of electrophoretic mobility shift assay, yeast one hybrid assay and dual-luciferase assay demonstrated that XsMYB30 could directly and specifically bind with the promoters of genes involved in wax biosynthesis (XsFAR4, XsCER1 and XsKCS1), lipid transfer (XsLTPG1 and XsLTP1) and fatty acid synthesis (XsKASIII), thus enhancing their expression. In addition, the overexpression of XsMYB30 in poplar promoted the expression levels of these target genes and increased the wax deposition on poplar leaves leading to a notable improvement in the plant's ability to withstand drought. These findings indicate that XsMYB30 is an important regulatory factor in cuticular wax biosynthesis and the drought resistance of yellowhorn.

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Plant Surface Properties and Treatments Plant Stress Responses and Tolerance Plant Water Relations and Carbon Dynamics

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DOI: 10.1093/treephys/tpae111

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