PRODUCTION OF RECOMBINANT β-GALACTOSIDASE PROTEIN IN BOMBYX MORI (BMN1) BACULOVIRUS INSECT CELLS
Shuhrat Sh. KHASANOVThe Institute of the Chemistry of Plant Substances, Tashkent, UzbekistanJumanazar Ch MENGTURAYEVTIIAME National Research University, Tashkent, UzbekistanJaloliddin AbdurakhmanovInstitute of the Chemistry of Plant Substances, Tashkent, UzbekistanDilmurodjon B. TURDALIYEVTIIAME National Research University, Tashkent, UzbekistanShakhnoza S. AzimovaInstitute of the Chemistry of Plant Substances, Tashkent, Uzbekistan
2025en
Ochiq kirishBronza
ABI
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In this study we aimed to obtain a novel recombinant β-galactosidase baculovirus using the Bombyx mori baculovirus – insect cell expression system. A new recombinant plasmid was constructed on the basis of the pBacPAK8 transfer vector and cloned in E. coli cells. Subsequently, a recombinant baculovirus was generated in Bombyx mori cells, and efficient expression of the β-galactosidase gene was observed. The enzymatic activity of the protein was confirmed by the ONPG assay. The results demonstrated that the baculovirus – insect cell system expands the possibilities for producing proteins of industrial significance.
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