The study of acute, subacute toxicity and cumulative properties of Bidens Frondosa L., harvested in Uzbekistan

Actuality.In the modern world, herbal medicines are used for the prevention and treatment of various diseases, along with a wide range of synthetic medicines.Currently, high quality standards are applied to all stages of the creation of pharmacological products, from research and development to implementation and production.Special attention is paid to ensuring the reliability of preclinical trials and ensuring the safety of new drugs.Bidens tripartita L. is a popular medicinal plant included in the State Register of Medicines of the Republic of Uzbekistan.However, due to the reduction of the natural habitats of the plant, the processing enterprises of the Republic began to experience a significant need for raw materials of this species, which is not yet cultivated in Uzbekistan.Due to the decrease in the area of growth, traditionally used in medical practice of the species of Bidens tripartita L., it was advisable to conduct a comprehensive pharmacognostic study of the Bidens frondosa L. species endemic to the Republic of Uzbekistan, which has significant reserves of medicinal plant raw materials.The aim.The objective of the research was to conducting pharmacological studies of acute and subacute toxicity and cumulative properties of Bidens frondosa L., harvested in Uzbekistan. Material and methods.The experiment was carried out according to the recommendations for conducting preclinical studies of medicines.Acute toxicity was studied in white mongrel mice, with a single injection of the test sample with the determination of LD 50 and toxicity class.Experiments to study subacute (subchronic) toxicity were carried out on 80 rats (40 males and 40 females) of the same age and body weight 170-200 g.The animals were kept in vivarium conditions with a standard diet (free access to water and feed), temperature and light conditions.All manipulations with animals were performed at the same time of day in the morning, taking into account the chronobiological dependence of most physiological processes in the body.The registration of biochemical blood parameters was carried out using standard reagent kits on a HumaLuzerPrimus 602828 biochemical analyzer, Germany.An experiment to study the cumulative properties of the medicinal plant Bidens frondosa L., was carried out according to the method of Lim R. et al. on 10 white mice weighing 20-22 g.Research results.As a result of the study of acute toxicity, it was found that the herb of the Bidens frondosa L., does not cause violations of the functional state of the main organs and systems of the body, and also does not have a general toxic effect at the studiedБіологія.Фармація doses and routes of administration.The data obtained from the study of subacute toxicity showed that after stopping the administration of herbal infusion of the Bidens frondosa L., death of rats was not observed in any of the groups.The observed changes in the clinical manifestations and behavioral reactions of mice during the experiment were reversible, and the animals were physiologically fully restored.No animal deaths were observed during the experiment. Conclusion.According to the classification of toxicity of substances, aboveground part of Bidens frondosa L., refers to practically non-toxic.In the study of subacute toxicity, the medicinal plant Bidens frondosa L., does not cause violations of the functional state of the main organs and systems of the body.It does not have a general toxic effect at the studied doses and routes of administration.The medicinal plant Bidens frondosa L., does not have cumulative properties.

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