STUDY OF THE POLYPHENOLIC COMPOSITION OF LEAVES OF THE SEMENOV MAPLE ACER SEMENOVII

For the first time, the chemical composition (polyphenols) of the leaves of the Semenov Maple plant - Acer semenovii - family Aceraceae Juss., growing in the mountainous regions of the Tashkent region of the Republic of Uzbekistan, has been studied. A study was carried out to study the yield of the amount of polyphenols using an optimized method of extraction from plant raw materials, depending on the composition of the extractant, the extraction module, the multiplicity of extraction, the ratio of raw materials: extractant, temperature, thickening conditions, treatment of the aqueous residue with organic solvents, the conditions for the precipitation of the amount of polyphenols and their drying. As a result, optimal conditions for the isolation of polyphenols from plant materials were selected and, under the selected conditions, the amount of polyphenols from the leaves of Maple Semenov was obtained with a yield of 8.9%. During a chromatographic study of the isolated fractions, it was found that the polyphenols of the ethyl acetate fraction are represented mainly by monomeric catechins, flavonols and tannins. Using physicochemical methods: paper chromatography, thin layer chromatography, ultraviolet spectroscopy, infrared spectroscopy and high-performance liquid chromatography, the structures of the isolated compounds were established. As a result, more than 14 polyphenols were isolated from the leaves of Maple Semenov, such as quercetin-3-O-β-D-glucopyranoside, rutin, kaempferol, quercetin, (+)-catechin, (-)-epicatechin, gallic acid, (-)- epigallocatechin gallate, 1-O-galloyl-β-D-glucose, procyanidin, ellagic acid, apigenin-6-C-glucoside, 1,6-bis-O-galloyl-β-D-glucose, isoquercetin.

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