COMPREHENSIVE ASSESSMENT OF THE IMPACT OF VARIOUS FACTORS ON THE QUALITY AND YIELD OF COLLAGEN

In this article, the optimal conditions for the extraction of collagen with its natural triple helical structure were studied by chemical hydrolysis of renewable raw materials -cattle hides.Changes in the amount of chemicals in the hide at different stages of hydrolysis were determined, and the optimal concentration of the alkaline-salt hydrolysis solution and the hydrolysis temperature were determined.According to the results of the study, when using a solution of 10% NaOH and 100 g/l Na 2 SO 4 at a temperature of 20 °C, natural collagen with a high yield of up to 83% was obtained.When the effect of the citrate buffer pH environment was studied, the pH=4 condition was found to be the most optimal.By measuring the UV spectrum of the isolated collagen, it was determined that it had a natural structure, and by finding the total nitrogen content, it was determined that it had a high purity level (99.6%).

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