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Behavioral and Synaptosomal Fluorescence Effects of Polyphenol in Hypothyroid Rats

K z k v IslomInstitute of Biophysics and Biochemistry at the National University of Uzbekistan , 100174 , Tashkent , UzbekistanKh shim v NozimInstitute of Biophysics and Biochemistry at the National University of Uzbekistan , 100174 , Tashkent , UzbekistanRaimova GuliInstitute of Biophysics and Biochemistry at the National University of Uzbekistan , 100174 , Tashkent , UzbekistanMukht r v AlisherNаtiоnаl Univеrsitу оf Uzbеkistаn named after M. Ulugbek , Tashkent , Almazar 100174 , UzbekistanM m d min v R hm tj nErkinov IslombekImpuls Medical Institute , 160114 , Namangan , UzbekistanMamatova ZulaykhoNаtiоnаl Univеrsitу оf Uzbеkistаn named after M. Ulugbek , Tashkent , Almazar 100174 , UzbekistanRakhimov RakhmatillaInstitute of Bioorganic Chemistry , 100125 , Tashkent , UzbekistanSafarova DilbarUzbek State University оf Physical Culture and Sport , 111709 , Chirchik , Uzbekistan

2026en

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ABI

Annotatsiya

Hypothyroidism alters behavior, endocrine homeostasis, and synaptic physiology in man and animals, yet presynaptic calciumassociated endpoints remain incompletely characterized.This study evaluated whether R-4, a Rhus glabra-derived polyphenolrich fraction, modulates endocrine, behavioral, biochemical, and synaptosomal calcium-associated responses in methimazoletreated rats.Adult male rats were assigned to control, hypothyroid, and hypothyroid + R-4 groups (n = 6 each).Hypothyroidism was induced with methimazole (2.5 mg/100 g/day, intragastrically) for 21 days, while R-4 was administered orally (50 mg/kg/day) during days 12-21.Open-field behavior, thyroid ultrasonography, serum thyroid hormones, serum and whole-brain biochemistry, synaptosome isolation, and Fluo-4-based fluorescence were assessed.Methimazole reduced T3, increased TSH, enlarged the thyroid gland, suppressed exploratory activity, and lowered basal synaptosomal Fluo-4-associated fluorescence relative to controls.R-4 partially normalized selected serum indices, improved exploratory behavior, and increased the reduced synaptosomal fluorescence signal in preparations from hypothyroid rats.The R-4-associated fluorescence response was attenuated under NMDA-related and voltage-gated Ca 2+-linked conditions, suggesting participation of both signaling domains.Because the readout was based on normalized Fluo-4 fluorescence rather than calibrated absolute [Ca 2+ ]i, the findings are interpreted as an altered synaptosomal calcium-associated fluorescence phenotype.Overall, the plant-derived Rhus glabra fraction behaved as a rapid synaptosome-level modulator in experimental hypothyroidism and supports further evaluation of plant bioactives in applied animal physiology.

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DOI: 10.17957/ijab/15.2493

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