THE COMPOSITION OF FLAVONOIDS OF THREE SPECIES OF THE GENUS SERRATULA

The composition of flavonoids in the above-ground part of Serratula species – S. manschurica Kitag., S. gmelinii Tausch., S. cupuliformis Nakai & Kitagawa, introduced in the Siberian Botanical Garden of Tomsk State University, was studied. Comparative HPLC analysis of extracts showed that S. cupuliformis is the most promising species in terms of flavonoid composition and content. The isolation of the sum of flavonoids from the above-ground part of Serratula cupuliformi by selective extraction and subsequent separation on columns allowed us to isolate an individual major flavonoid. Using HPLC-MS methods, it was identified as 5,7,2',4'-tetrahydroxy-3-methoxyflavone; the presence of a number of other flavonoids was established: isosilichristin, resokaempferol, amorphigenin glucoside, etc. An analysis of the literature data showed that 5,7,2',4'-tetrahydroxy-3-methoxyflavone was identified for the first time in the species of the genus Serratula. The results of the analysis of the flavonoid content during the vegetative development of the studied species indicate that the phase of maximum flavonoid accumulation is the periods of active plant growth - the beginning of the vegetation period for S. cupuliformis, flowering for S. manshurica and S. gmelinii. It is noted that during the vegetative development of S. cupuliformis, some flavonoids (5,7,2',4'-tetrahydroxy-3-methoxyflavone) are found in all phases of vegetation, while the presence of others is established in certain periods of development. In all likelihood, such distribution and selective synthesis of individual flavonoids is due to the needs of plants and the participation of phenolic compounds in certain stages of metabolism.

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