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Protective Effects of Polyphenol Preparations from Vitis vinifera, Punica granatum, and Atraphaxis pyrifolia in an L-Arginine-Induced Rat Model of Acute Pancreatitis: Biochemical and Histopathological Evaluation

Bibimaryam Muratbayeva3Department of Human and Animal Physiology, Faculty of Biology and Ecology, National University of Uzbekistan, UzbekistanQo‘zijon Baratov1Department of Pharmacology and Proteins and Peptides, Institute of Bioorganic Chemistry named after A.S.Sadykov, Tashkent, UzbekistanZulaykho MAMATOVA3Department of Human and Animal Physiology, Faculty of Biology and Ecology, National University of Uzbekistan, UzbekistanNurali Ergashev2Department of Molecular Biophysics, Institute of Biophysics and Biochemistry, UzbekistanRakhmonova Gulnora1Department of Pharmacology and Proteins and Peptides, Institute of Bioorganic Chemistry named after A.S.Sadykov, Tashkent, UzbekistanZulfizar Kuziyeva1Department of Pharmacology and Proteins and Peptides, Institute of Bioorganic Chemistry named after A.S.Sadykov, Tashkent, UzbekistanNodira Abdulladjanova1Department of Pharmacology and Proteins and Peptides, Institute of Bioorganic Chemistry named after A.S.Sadykov, Tashkent, UzbekistanSherali Kuziev4Department of Biochemistry, National University of Uzbekistan, UzbekistanKamola Vakhabdzhanovna Raimova1Department of Pharmacology and Proteins and Peptides, Institute of Bioorganic Chemistry named after A.S.Sadykov, Tashkent, UzbekistanVohidjon Nurboyev3Department of Human and Animal Physiology, Faculty of Biology and Ecology, National University of Uzbekistan, Uzbekistan

2026

Ochiq kirishOlmos

Litsenziya: cc-by

Manbada oʻqishdoi.org(yangi oynada ochiladi)

ABI

Annotatsiya

Acute pancreatitis is accompanied by pancreatic tissue injury, systemic inflammatory responses, metabolic disturbances, and secondary hepatic dysfunction. This study evaluated the protective effects of selected polyphenol preparations in an L-arginine-induced rat model of acute pancreatitis using biochemical and histopathological approaches. Acute pancreatitis was induced in white laboratory rats by intraperitoneal administration of L-arginine hydrochloride at 2000 mg/kg twice with a 1-hour interval. After model induction, animals were treated orally for 21 days with Punitan, Providin, and Kurchavka at doses of 50 and 100 mg/kg, while resveratrol 30 mg/kg was used as a reference compound. Serum total protein, glucose, ALT, AST, α-amylase, alkaline phosphatase, total cholesterol, total bilirubin, and lipase were determined. Pancreatic tissues were examined after hematoxylin–eosin staining. Untreated pancreatitis animals showed increased ALT, AST, α-amylase, total bilirubin, and lipase levels, indicating pancreatic injury and hepatic dysfunction. Punitan most effectively normalized total protein, α-amylase, and bilirubin levels, suggesting a pronounced pancreatoprotective effect. Providin markedly reduced ALT and AST activities, indicating stronger hepatoprotective potential. Kurchavka improved cholesterol levels, but its effect on pancreatic enzymes was limited. Histological examination revealed edema, inflammatory infiltration, acinar disorganization, dystrophic changes, atrophy, and lipomatous alterations in pancreatitis groups. Lower doses, especially Providin 50 mg/kg, showed milder and mostly reversible morphological changes. These findings indicate that the studied polyphenol preparations exert preparation-specific and dose-dependent protective effects against L-arginine-induced acute pancreatitis.

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Identifikatorlar

DOI: 10.13005/bpj/3534

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