TECHNOLOGICAL ASPECTS OF THE PRODUCTION OF SKIMMIANINE FROM THE AERIAL PART OF HAPLOPHYLLUM PERFORATUM

To purify skimmianine from accompanying substances, optimal conditions were selected for the purification process of the extract obtained from the aerial part of Haplophyllum perforatum through extraction with 80% ethyl alcohol. A process of settling the distillation residue for 24 hours after concentrating the extract to 10% of its initial volume and acidifying it to pH 5 was proposed for the precipitation of phenolic compounds. It was determined that after the removal of phenolic compounds, three successive treatments of the distillation residue at pH 5 with chloroform are sufficient to eliminate hydrophobic accompanying substances, followed by five sequential extractions of skimmianine from the aqueous alkaloid solution at pH 10 using chloroform. To remove minor alkaloids, the alkaloid mixture was treated with acetone at a 1 : 5 ratio under boiling conditions, ensuring the production of technical skimmianine with a purity of 92%. It was found that recrystallization of technical skimmianine from a methanol-chloroform mixture in a 1 : 3 volume ratio allows for obtaining skimmianine with a purity of at least 99%. Based on the obtained results, a technological scheme for the production of skimmianine from the aerial part of Haplophyllum perforatum was developed, achieving an 80% yield of skimmianine from the raw material.

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