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Effect of Kaempferol on the KATP-channel and PTP in rat liver Mitochondria

Yuldoshev BPhD Student of Institute of Biophysics and Biochemistry at the National University of Uzbekistan, Tashkent, Republic of UzbekistanErgashev N.DSc, Senior Researcher, Institute of Biophysics and Biochemistryat the National University of Uzbekistan, Tashkent, Republic of UzbekistanKomilov E.PhD in Biological Sciences, Senior Researcher, Institute of Biophysics and Biochemistry at the National University of UzbekistanAbdulkhakova G.PhD Student of Institute of Biophysics and Biochemistry at the National University of Uzbekistan, Tashkent, Republic of UzbekistanAbdullaev I.Institute of Bioorganic Chemistry named after A.Sadykov, Tashkent, UzbekistanGayibov UInstitute of Bioorganic Chemistry named after A.Sadykov, Tashkent, UzbekistanAsrarov M.Professor of the Institute of Biophysics and Biochemistry at the National University of Uzbekistan, DSc. UzbekistanRakhmatillayeva Y.Master’s student of Karshi state university, Karshi, Republic of UzbekistanPayziyeva O.Ferghana Polytechnical Institute

2026

Ochiq kirish: yoʻq

ABI

Annotatsiya

Free radicals formed from unsaturated fatty acids as a result of lipoperoxidation have been shown to act as secondary messengers in the oxidative/electrophilic stress process by transmitting electrophilic signals, as well as participating in key cellular processes such as autophagy, proliferation, and apoptosis through modulation of fundamental intracellular pathways. It has also been shown that electron transport in the mitochondrial respiratory chain, ion transport processes, Ca²⁺ ion homeostasis, and other cellular metabolic processes are closely interconnected with lipoperoxidation processes. In recent years, biologically active compounds of plant origin have been extensively utilized in the prevention of oxidative processes occurring in the body. Accordingly, we considered it important to investigate the mechanism of action of the flavonoid kaempferol (3,4',5,7-tetrahydroxyflavone) in vivo and in vitro studies. In addition, the effect of the kaempferol on ATP-sensitive potassium channel activity and the state of the mitochondrial permeability transition pore (mPTP) in rat liver mitochondria under alloxan-induced oxidative stress was investigated using a spectrophotometric method in in vivo studies. The conducted studies revealed that a dose of 50mg/kg of the flavonoid exhibited stronger antioxidant activity under oxidative stress conditions compared to the 10mg/kg dose. In this case, animals administered 50mg/kg of kaempferol relative to their body weight exhibited ATP-sensitive potassium channel activity that was 3.02±0.3 times higher compared to the mitoKATP-channel activity of the experimental animals. Kaempferol demonstrated a significant capacity to suppress the opening of the mitochondrial permeability transition pore (mPTP), reducing mitochondrial swelling by approximately 68.49±3.1% under conditions of oxidative stress. These findings suggest that, beyond its notable antioxidant potential, this flavonoid effectively prevents mPTP activation an event implicated in various physiological and pathological mechanisms and also enhances the function of мitоKАTР-сhаnnеl.

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Identifikatorlar

DOI: 10.52711/0974-360x.2026.00676

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