DETERMINATION OF AFLATOXIN (AF) AND OCHRATOXIN A (OTA) PRODUCTION BY ASPERGILLUS FLAVUS STRAIN

Aflatoxins (AF) are a group of secondary metabolites belonging to the group of furanocoumarins synthesized by mycotoxigenic micromycetes of the genus Aspergillus. The presence of micromycetes of the genus Aspergillus in animal feed, the production of secondary metabolites, AF is a global problem that negatively affects animal and human health, causing significant economic losses. This study is aimed at determining the microflora of animal feed in Uzbekistan, identifying micromycetes of the genus Aspergillus based on morphological and molecular methods. Mycotoxin production and genes involved in mycotoxin production were determined using PCR amplification of idh genes of mycotoxigenic micromycetes, Immunofermentative assay (IFA), HPLC-MS methods.

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