EXPERIMENTAL DETERMINATION OF THE EFFECTIVE DOSE RANGE OF A MULTICOMPONENT SEDATIVE HERBAL TEA

The development of herbal sedative products requires comprehensive evaluation of efficacy, safety, dose–response relationships, and the persistence of effects during repeated administration. Objective. To compare the neuropharmacological activity and acute toxicity of Somnilaks NIIVS herbal tea with those of Pustyrnik herbal tea. Materials and methods. Experiments were conducted in white mice weighing 18–22 g. Acute toxicity was evaluated following a single intragastric administration at doses of 1,000–15,000 mg/kg. Sedative activity was assessed at 1–200 mg/kg in the open-field test, by measurements of locomotor and vertical activity, and under phenamine-induced stimulation after single, 10-day, and 20-day administration. Results. The LD₅₀ values of both herbal teas exceeded 15,000 mg/kg. The greatest reductions in motor and exploratory activity were generally observed at doses of 25–50 mg/kg. Following a single administration, Pustyrnik showed a stronger immediate effect, whereas Somnilaks NIIVS had a more pronounced and sustained effect after a 10-day course. By day 20, the effects of the two products became comparable. Neither product completely abolished phenamine-induced locomotor stimulation, although exploratory activity was significantly reduced. Conclusion. Somnilaks NIIVS demonstrated a favourable experimental safety profile and persistent sedative activity during repeated administration, supporting further preclinical investigation. Keywords: herbal tea, sedative aivity, acute toxicity, open-field test, locomotor activity, phenamine, Somnilaks NIIVS.

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