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Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells

Junying YuGenome Center of Wisconsin, Madison, WI 53706–1580, USAMaxim A. VodyanikGenome Center of Wisconsin, Madison, WI 53706–1580, USAKim Smuga-OttoDepartment of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, WI 53706, USAJessica Antosiewicz‐BourgetGenome Center of Wisconsin, Madison, WI 53706–1580, USAJennifer L. FraneGenome Center of Wisconsin, Madison, WI 53706–1580, USAShulan TianDepartment of Anatomy, University of Wisconsin-Madison, Madison, WI 53706–1509, USAJeff NieDepartment of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, WI 53706, USAGuðrún A. JónsdóttirDepartment of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, WI 53706, USAVictor RuottiDepartment of Anatomy, University of Wisconsin-Madison, Madison, WI 53706–1509, USARon StewartGenome Center of Wisconsin, Madison, WI 53706–1580, USAIgor I. SlukvinDepartment of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, WI 53706, USAJames A. ThomsonDepartment of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, WI 53706, USA

2007en

Ochiq kirish: yoʻq

ABI

Annotatsiya

Somatic cell nuclear transfer allows trans-acting factors present in the mammalian oocyte to reprogram somatic cell nuclei to an undifferentiated state. We show that four factors (OCT4, SOX2, NANOG, and LIN28) are sufficient to reprogram human somatic cells to pluripotent stem cells that exhibit the essential characteristics of embryonic stem (ES) cells. These induced pluripotent human stem cells have normal karyotypes, express telomerase activity, express cell surface markers and genes that characterize human ES cells, and maintain the developmental potential to differentiate into advanced derivatives of all three primary germ layers. Such induced pluripotent human cell lines should be useful in the production of new disease models and in drug development, as well as for applications in transplantation medicine, once technical limitations (for example, mutation through viral integration) are eliminated.

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Identifikatorlar

DOI: 10.1126/science.1151526

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