Naked-Eye Detection of Grapevine Red-Blotch Viral Infection Using a Plasmonic CRISPR Cas12a Assay
Yongya LiDepartment of Chemistry, Centre for Biotechnology, Brock University, St. Catharines, Ontario L2S 3A1, CanadaHayam MansourDepartment of Chemistry, Centre for Biotechnology, Brock University, St. Catharines, Ontario L2S 3A1, CanadaTony WangCool Climate Oenology and Viticulture Institute, Brock University, St. Catharines, Ontario L2S 3A1, CanadaSudarsana PoojariCool Climate Oenology and Viticulture Institute, Brock University, St. Catharines, Ontario L2S 3A1, CanadaFeng LiCollege of Chemistry, Sichuan University, Chengdu, Sichuan 610064, P.R. China
2019en
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ABI
Annotatsiya
Herein, we described a novel plasmonic CRISPR Cas12a assay for the visual, colorimetric detection of grapevine viral infections. Our assay generates rapid and specific colorimetric signals for nucleic acid amplicons by combining the unique target-induced incriminate single-stranded DNase activity of Cas12a with plasmon coupling of DNA functionalized gold nanoparticles. The practical applicability of our plasmonic assay was successfully demonstrated through the detection of emerging red-blotch viral infections in grapevine samples collected from commercial vineyards.
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