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Visualization of Zika Virus Infection via a Light-Initiated Bio-Orthogonal Cycloaddition Labeling Strategy

Judun ZhengMolecular Diagnosis and Treatment Center for Infectious Diseases, Dermatology Hospital, Southern Medical University, Guangzhou, ChinaRui YueMolecular Diagnosis and Treatment Center for Infectious Diseases, Dermatology Hospital, Southern Medical University, Guangzhou, ChinaRonghua YangDepartment of Burn and Plastic Surgery, Guangzhou First People's Hospital, South China University of Technology, Guangzhou, ChinaQikang WuDepartment of Burn Surgery & Department of Rheumatology, The First People's Hospital of Foshan, Foshan, ChinaYunxia WuDepartment of Burn Surgery & Department of Rheumatology, The First People's Hospital of Foshan, Foshan, ChinaMingxing HuangDepartment of Infectious Disease, the Fifth Affiliated Hospital, Sun Yat-sen University, Zhuhai, ChinaXu ChenDepartment of Burn Surgery & Department of Rheumatology, The First People's Hospital of Foshan, Foshan, ChinaWeiqiang LinMolecular Diagnosis and Treatment Center for Infectious Diseases, Dermatology Hospital, Southern Medical University, Guangzhou, ChinaJialin HuangDepartment of Burn Surgery & Department of Rheumatology, The First People’s Hospital of Foshan, Foshan, ChinaXiaodong ChenDepartment of Clinical Laboratory, The First People's Hospital of Foshan, Foshan, ChinaXiaodong ChenDepartment of Burn Surgery & Department of Rheumatology, The First People's Hospital of Foshan, Foshan, ChinaYideng JiangNHC Key Laboratory of Metabolic Cardiovascular Diseases Research, Ningxia Key Laboratory of Vascular Injury and Repair Research, Ningxia Medical University, Yinchuan, ChinaBin YangMolecular Diagnosis and Treatment Center for Infectious Diseases, Dermatology Hospital, Southern Medical University, Guangzhou, ChinaYuhui LiaoSouthern Medical University

2022en

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Litsenziya: cc-by

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Annotatsiya

Zika virus (ZIKV) is a re-emerging flavivirus that leads to devastating consequences for fetal development. It is crucial to visualize the pathogenicity activities of ZIKV ranging from infection pathways to immunity processes, but the accurate labeling of ZIKV remains challenging due to the lack of a reliable labeling technique. We introduce the photo-activated bio-orthogonal cycloaddition to construct a fluorogenic probe for the labeling and visualizing of ZIKV. Via a simple UV photoirradiation, the fluorogenic probes could be effectively labeled on the ZIKV. We demonstrated that it can be used for investigating the interaction between ZIKV and diverse cells and avoiding the autofluorescence phenomenon in traditional immunofluorescence assay. Thus, this bioorthogonal-enabled labeling strategy can serve as a promising approach to monitor and understand the interaction between the ZIKV and host cells.

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Identifikatorlar

DOI: 10.3389/fbioe.2022.940511

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