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BUSCO: assessing genome assembly and annotation completeness with single-copy orthologs

Felipe A. SimãoSIB Swiss Institute of BioinformaticsRobert M. WaterhouseSIB Swiss Institute of BioinformaticsPanagiotis IoannidisSIB Swiss Institute of BioinformaticsEvgenia V. KriventsevaDepartment of Genetic Medicine and Development, University of Geneva Medical School and Swiss Institute of Bioinformatics, rue Michel-Servet 1, 1211 Geneva, SwitzerlandEvgeny M. ZdobnovSIB Swiss Institute of Bioinformatics

2015en

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MOTIVATION: Genomics has revolutionized biological research, but quality assessment of the resulting assembled sequences is complicated and remains mostly limited to technical measures like N50. RESULTS: We propose a measure for quantitative assessment of genome assembly and annotation completeness based on evolutionarily informed expectations of gene content. We implemented the assessment procedure in open-source software, with sets of Benchmarking Universal Single-Copy Orthologs, named BUSCO. AVAILABILITY AND IMPLEMENTATION: Software implemented in Python and datasets available for download from http://busco.ezlab.org. CONTACT: [email protected] SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.

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DOI: 10.1093/bioinformatics/btv351

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