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Connexins regulate calcium signaling by controlling ATP release

Maria Luisa CotrinaNew York Medical CollegeJane H.-C. LinNew York Medical CollegeAlexandra Alves‐RodriguesDepartments of Cell Biology and Anatomy, Pathology, and Neurosurgery, New York Medical College, Valhalla, NY 10595; and Department of Anatomy and Cell Biology, University of Western Ontario, London, Ontario N6A 5C1, CanadaShujun LiuNew York Medical CollegeJiang LiNew York Medical CollegeHooman Azmi-GhadimiNew York Medical CollegeJian KangDepartments of Cell Biology and Anatomy, Pathology, and Neurosurgery, New York Medical College, Valhalla, NY 10595; and Department of Anatomy and Cell Biology, University of Western Ontario, London, Ontario N6A 5C1, CanadaChristian C. NausNew York Medical CollegeMaiken NedergaardDepartments of Cell Biology and Anatomy, Pathology, and Neurosurgery, New York Medical College, Valhalla, NY 10595; and Department of Anatomy and Cell Biology, University of Western Ontario, London, Ontario N6A 5C1, Canada
1998en
Ochiq kirishYashil
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Annotatsiya

Forced expression of gap junction proteins, connexins, enables gap junction-deficient cell lines to propagate intercellular calcium waves. Here, we show that ATP secretion from the poorly coupled cell lines, C6 glioma, HeLa, and U373 glioblastoma, is potentiated 5- to 15-fold by connexin expression. ATP release required purinergic receptor-activated intracellular Ca2+ mobilization and was inhibited by Cl- channel blockers. Calcium wave propagation also was reduced by purinergic receptor antagonists and by Cl- channel blockers but insensitive to gap junction inhibitors. These observations suggest that cell-to-cell signaling associated with connexin expression results from enhanced ATP release and not, as previously believed, from an increase in intercellular coupling.

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DOI
10.1073/pnas.95.26.15735

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