Adventitious Roots' Induction of Pinus massoniana Shoots in Test Tubes and Anatomical Observation

Adventitious roots induction for Pinus massoniana from seed source in vitro was investigated,including the influences of different media on the induction by using L16(45) orthogonal design.The results showed that the best medium was 1/3DCR+NAA0.15 mg·L-1+sucrose 2%+agar 0.6%,the highest rate of rooting was 51.7%,with many fibrous roots,and high quality.Meanwhile,the adventitious root formation of shoots in test tube was also observed by means of the anatomical method.Latent root primordium was not found in the shoots in test tubes.The induced root primordium which originated from the division and differentiation of medullary ray cell developed into the adventitious roots.

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