Nukus shahridan olingan Melilotus officinalis (L.) Lam. namunasining xloroplast matK geni ketma-ketligi asosida molekulyar-genetik identifikatsiyasi

The article presents the results of molecular genetic identification of yellow sweet clover, Melilotus officinalis (L.) Lam., growing in the city of Nukus, Republic of Karakalpakstan. To confirm the species identity of the studied sample, a fragment of the maturase K (matK) gene of the chloroplast genome was used. The nucleotide sequence was obtained by Sanger sequencing.The analysed nucleotide sequence was 499 base pairs (bp) long. Genomic DNA was extracted from a plant sample collected in Nukus on 20 May 2026. The sequence contained 148 adenine (A), 76 cytosine (C), 66 guanine (G) and 209 thymine (T) nucleotides. The GC content was 28.46 % and the AT content 71.54 %.It was shown that the obtained matK gene fragment can be used as an additional marker for molecular confirmation of the taxonomic identity of the Melilotus officinalis sample. The nucleotide sequence has been prepared for deposition in the international GenBank/NCBI database.

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