Enzymatic hydrolysis of goat (Capra hircus) muscle proteins: from hydrolysates and peptides to digestibility and functional food ingredients - protocol for a systematic review

eview question / Objective Primary question.In studies of goat (Capra hircus) skeletal-muscle proteins, how do the type of protease and the hydrolysis conditions (pH, temperature, time, enzyme-to-substrate ratio, thermal pretreatment), as well as simulated gastrointestinal digestion, affect the degree of hydrolysis (DH), molecular weight distribution, peptide and amino-acid profile, digestibility, techno-functional properties and bioactivity of the resulting hydrolysates and peptides?Framework (PICOS adapted for food technology).P (substrate): skeletal muscle of Capra hircusmeat, primal cuts, myofibrillar or sarcoplasmic protein fractions, meat extracts, raw or heattreated.I (process): hydrolysis with exogenous proteases (commercial, plant, animal or microbial), single or sequential, and simulated gastrointestinal digestion.C (comparator): unhydrolysed protein; other enzymes, doses, times or temperatures within the same study; where available, the same process applied to meat of other species in the same experiment.O (outcomes): DH, molecular weight distribution, peptide sequences, aminoacid profile, in vitro/in vivo digestibility, technofunctional properties, bioactivity (ACE, DPP-IV, antioxidant, antimicrobial, anti-inflammatory) and performance in food matrices.S (study design): in vitro, cell-culture, animal and pilot technological studies.Secondary objectives.(i) To appraise the methodological quality and reporting of the evidence; (ii) to grade the level of evidence for bioactivity from crude hydrolysate to in vivo; (iii) to judge whether quantitative synthesis is feasible; (iv) to identify research gaps relevant to the development of goat-meat hydrolysates as ingredients of specialised (including gerodietetic) meat products.Rationale Goat meat is a lean red meat with a high protein content and a favourable fatty-acid profile, and Kazakhstan has one of the largest smallruminant populations in Central Asia, much of it kept in households.Yet goat meat is rarely INPLASY 1 International Platform of Registered Systematic Review and Meta-analysis Protocols INPLASY Enzymatic hydrolysis of goat (Capra hircus) muscle proteins: from hydrolysates and peptides to digestibility and functional food ingredientsprotocol for a systematic review Mukhanbetova, N; Makangali, K; Iskakova, SA.

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