Time dependent viscoelastic image of living nerve cells using AFM
Kazushige KawabataDivisions of PhysicsHanae IshizukaDivisions of PhysicsT. NittaDivisions of PhysicsHisashi HagaDivisions of PhysicsEtsuro ItoBiological Sciences, Graduate School of Science, Hokkaido Univ., Sapporo 060-0810, JapanKazuhiro AbeDepartment of Anatomy, Graduate School of Medicine, Hokkaido Univ., Sapporo 060-8638, JapanTatsuo UshikiDepartment of Anatomy, Niigata Univ. School of Medicine, Niigata 951-8510, JapanTakashi SambongiDivisions of Physics
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The topographic and viscoelastic images of the nerve cell (NG108-15) living in the culture medium were successfully obtained in the force modulation mode using atomic force microscopy (AFM). We found that there exists variation of elasticity in the cell. To compare the elastic results with F-actin network of cytoskeleton which is considered as a possible origin for cell stiffness, the fluorescence observation was made on the fixed cells just after the AFM measurements. The results show no clear correspondence between density of F-actin and stiffness of the cells.
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