Measurement of the operation frequency of reaction centers in plant leaves by laser correlation spectroscopy
V. S. AgishevInstitute of Physiology and Biophysics, Academy of Sciences of Uzbekistan, Tashkent, 700096, UzbekistanI. A. KhusainovNPO Akadempribor, Academy of Sciences of Uzbekistan, Tashkent, 700125, UzbekistanA. V. ZinovevNPO ‘Akadempribor’, Academy of Sciences of Uzbekistan, Tashkent, UzbekistanT. UsmanovNPO Akadempribor, Academy of Sciences of Uzbekistan, Tashkent, 700125, Uzbekistan
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The operation frequency of a reaction center of photosystem 2 in an intact leaf has been determined for the first time by the method of correlation analysis. The leaf was illuminated by radiation from a He-Ne laser. Fluorescence was measured at a wavelength of 685 nm. Processing of the experimental data showed that the frequency spectrum of the spectral density of the intensity of fluorescence of the leaf exhibits a peak at a frequency of 1670±50 Hz. This frequency corresponds to a time interval of ∼0.5 ms, which coincides with the time of diffusion of plastohydroquinone in the lipid layer of the thylakoid membrane.
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