Phenol biodegradation by a Pseudomonas sp. strain tagged with the gfp gene
Aima AdylovaInstitute of Plant Genetics and Experimental Biology, Academy of Sciences of Uzbekistan, Yukori-Yuz Village, Tashkent oblast’, 702151, UzbekistanTatyana N. ChernikovaInstitute of Plant Genetics and Experimental Biology, Academy of Sciences of Uzbekistan, Yukori-Yuz Village, Tashkent oblast’, 702151, UzbekistanAbdusattor AbdukarimovInstitute of Plant Genetics and Experimental Biology, Academy of Sciences of Uzbekistan, Yukori-Yuz Village, Tashkent oblast’, 702151, Uzbekistan
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Conjugal transfer of the pAG408 suicide vector from E. coli S 17.1 to Pseudomonas sp. cells able to consume phenol yielded transconjugates brightly luminescing under UV illumination. It was shown that tagging of the Pseudomonas sp. cells with the gfp gene did not affect their ability to consume phenol. The change of the population density of the tagged bacteria after their introduction to soil was studied. The potential of the resulting bacterial strain in remediation of phenol-polluted soils is discussed.
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